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-   -   Single-cell RNA-seq normalization with ERCC (http://seqanswers.com/forums/showthread.php?t=72892)

kobeho24 12-03-2016 01:24 AM

Single-cell RNA-seq normalization with ERCC
 
Hi everyone,
Does anyone have any recommendation on the single-cell rna-seq data normalization methods? I would like to do normalization across a mix population of cells, in particular, transcript-level analysis is demanded. I know there are a whole bunch of methods doing well on gene-level, just wonder how can we do the similar thing on isoform-level with ERCC as spike-ins.

BTW, I intended to use alighment-free quantification programs such as kallisto or salmon, since I have a hugh dataset and they are much faster and require less computational resources.

Thanks in advance!

Wish you all a merry Christmas and a happy new year:)

Gary


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