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kaps 05-31-2015 11:52 PM

bioedit vs blastn results
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hello, I have blasted my contigs and obtained some hits with high percentage identities. I then retrieved the fasta files of those hits and computed sequence identity matrix using bioedit. I expected the results from both blastn and bioedit to be the same, but they are not. Is this proper? if Not, what could be the problem?

I have attached a doc showing results

GenoMax 06-01-2015 04:32 AM

What exactly are you trying to do?

Blastn hits are probably not full length (unless the sequences are very homologous from end-to-end). I assume you are importing sequences that are full length into bioedit and then aligning them with clustalW (which is now a global alignment instead of the local in Blast). That is probably the reason why your identity matrix is not showing values closer to 1.0.

kaps 06-01-2015 07:02 AM


My interest is to generate a Pairwise sequence comparison matrix.
the blastn hits include both full length and partial. Seq identity matrix I posted were generated without clustalw alignment. when I aligned only 3 long seqs out of the 12 seqs remained. Imagine the 9 were eliminated because of being short?,dissimilar?

GenoMax 06-01-2015 07:18 AM

Are these sequences of similar length? Are there repeats (internal)?

You may want to look at dot plots if you are trying to find similarities.

kaps 06-02-2015 06:37 AM

The sequences differ in length. I have not checked whether there are repeats. how do I check for repeats?

I will read about dot plots using the link provided.


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