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-   -   Difference when shearing with Low-EDTA and Water? (http://seqanswers.com/forums/showthread.php?t=66299)

wangwde 02-15-2016 04:02 AM

Difference when shearing with Low-EDTA and Water?
 
Dear all,
Does anyone have ever test the shearing protocol provided by Covaris with different buffer? e.g. low-EDTA buffer and Water?
thank you!
Wei

nucacidhunter 02-15-2016 05:05 PM

TE buffer which has been recommended is the best option. For fragmentation to small sizes 250bp< degradation level increases if water is used for resuspension of DNA.

neurula 02-17-2016 03:36 PM

I haven't compared all different buffer types but we routinely use TE - low EDTA (10 mM Tris-HCl, pH 8.0, 0.1 mM EDTA) with excellent results, fragmenting to ~160bp.


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