SEQanswers (
-   Bioinformatics (
-   -   How to specify/calculate subject coverage in tBLASTn (

dineshkumarsrk 03-16-2019 01:26 AM

How to specify/calculate subject coverage in tBLASTn
I have been screening the homologous gene sequences from my genome of interest by doing tBLASTn, wherein I have to specify two mandatory parameters such as,
Coverage = 40% and Percentage of identity = 70%
Percentage of identity can be specified or derived from the tabular out put format (-outfmt 6). Since tBLASTn analyse, protein against nucleotide hits, therefore how to calculate the coverage percentage of the same. if i manually calculate the coverage percentage, its so tedious (3 nucleotide to 1 amino acid formula) process. Moreover, I have to carry out this analysis for numerous genomes. Hence, please suggest me any strategy to do the same.
Thank you in advance:)

All times are GMT -8. The time now is 08:10 AM.

Powered by vBulletin® Version 3.8.9
Copyright ©2000 - 2020, vBulletin Solutions, Inc.