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-   -   Recommended pipeline for differential miRNA-seq (http://seqanswers.com/forums/showthread.php?t=74170)

AlexClop 02-10-2017 07:32 AM

Recommended pipeline for differential miRNA-seq
 
Dear all,

I was a SeqAnswers member few years ago while working on human genetics at Kings' College London. I just re-registered again, in my current job as animal geneticist at CRAG (Barcelona).

We want to carry a microRNA-seq experiment in an Illumina HiSeq2000 to carry a differential expression analysis from porcine sperm.

I would like to know which is the most recommendable bioinformatics pipeline nowadays for our miRNA-seq study.

Thanks for sharing your knowledge and experience!

Alex Clop

sbarberan 03-08-2017 11:22 PM

I use CAP-miRSeq that includes differential expression analysis with edgeR

http://bioinformaticstools.mayo.edu/...ch/cap-mirseq/


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