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Hunny 04-18-2011 03:38 PM

Where could I get a set of real RNA-Seq data
 
Hello guys!

I would like to know where I could get a set of real RNA-Seq data.

I've searched many papers about RNA-Seq, but I haven't found any one providing RNA-Seq data.

Thanks :)

d17 04-18-2011 03:58 PM

Try taking a look at this paper:
http://www.nature.com/nature/journal...ture08872.html

Their data is available on their website and it is pretty easy to access.

Hunny 04-18-2011 04:05 PM

Quote:

Originally Posted by d17 (Post 39795)
Try taking a look at this paper:
http://www.nature.com/nature/journal...ture08872.html

Their data is available on their website and it is pretty easy to access.

Thank you so much. I will check it :)

Hunny 04-18-2011 05:21 PM

Quote:

Originally Posted by d17 (Post 39795)
Try taking a look at this paper:
http://www.nature.com/nature/journal...ture08872.html

Their data is available on their website and it is pretty easy to access.

Hi,

Thank you again! I have downloaded the data from their website successfully!

Since I want to test TopHat using a real dataset, I also need a reference genome for mapping the RNA-Seq data above.

So, How could I get a reference genome?

Thanks :)

jstjohn 04-18-2011 06:47 PM

http://www.ncbi.nlm.nih.gov/projects...an/index.shtml

wuj 04-19-2011 11:57 AM

Quote:

Originally Posted by Hunny (Post 39803)
Hi,

Thank you again! I have downloaded the data from their website successfully!

Since I want to test TopHat using a real dataset, I also need a reference genome for mapping the RNA-Seq data above.

So, How could I get a reference genome?

Thanks :)

You need to get bowtie first, and....perhaps you need to read manuals of bowtie and tophat before you actually start....

FYI: reference genome sequences are available on UCSC genome browser -> download


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