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-   -   rRNA sizes on Agarose gel (http://seqanswers.com/forums/showthread.php?t=32041)

lior lobel 07-17-2013 08:14 AM

rRNA sizes on Agarose gel
 
1 Attachment(s)
Hi All,

I ran a Agarsoe gel with my RNA samples and got the attached pattern.
23S rRNA and 16S rRNA are supposed to be ~3000 bps and ~1500 bps, however in this run I see 1500 and 750 bands.

Can this be a gel problem? Has anyone encountered this?

Thanks,

Lior

cliffbeall 07-19-2013 05:21 AM

RNA will not run on a gel exactly according to its size because it is single stranded and the secondary structure can be variable. To get an accurate size you need to use a denaturing gel (formaldehyde, urea, methyl mercury) and compare it to RNA standards.

Since you see 2 pretty sharp bands, your RNA is probably fine.

Edit: There is also a bioanalyzer chip for RNA that would be an option if you have access to an instrument.


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