SEQanswers

SEQanswers (http://seqanswers.com/forums/index.php)
-   Sample Prep / Library Generation (http://seqanswers.com/forums/forumdisplay.php?f=25)
-   -   Please help! DNA samples for whole genome sequencing (http://seqanswers.com/forums/showthread.php?t=70993)

Vinn 08-21-2016 10:26 AM

Please help! DNA samples for whole genome sequencing
 
Hi folks,

I have extracted DNA from fungi for whole genome sequencing (Truseq PCR free with 350 bp insert and HiSeq 2500 PE) using a kit. The extraction went smooth until I realized at the end that I treated my samples with only half of the recommended amount for RNase A (2 ul of 10 mg/ml instead of 2 ul of 20 mg/ml). I tried checking the DNA samples on 1% agarose and following is the gel image with 100-1000 bp ladder.


https://s9.postimg.org/trlhi1ugv/UVI00239.jpg


I am not sure there are RNA bands (very faint though) at around 100-200 bp. Do you think my DNA samples are good enough to go for whole genome sequencing without additional treatments? Or shall I try to treat them with RNase A again? Unfortunately, I run out of fresh samples. :(

I appreciate all your comments and suggestions.

Best
Vinn

jdk787 08-21-2016 10:40 AM

These look fine. Even if you have some RNA in your sample it won't make into your final library after fragmentation and ligation steps.


All times are GMT -8. The time now is 05:09 AM.

Powered by vBulletin® Version 3.8.9
Copyright ©2000 - 2020, vBulletin Solutions, Inc.