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-   -   picard error: Mismatch between read length and quals length writing read (http://seqanswers.com/forums/showthread.php?t=22641)

shawpa 08-20-2012 06:52 AM

picard error: Mismatch between read length and quals length writing read
 
I am getting an error in picard when trying to merge some sam files. I can't find this error posted about anywhere. I am wondering if someone knows what it might mean and how i can go about fixing it. The output for me standard error is:

Exception in thread "main" java.lang.RuntimeException: Mismatch between read length and quals length writing read MWR-PRG-0014:86:D13KTACXX:4:2203:11277:100870_1:N:0:CTTGTA/1; read length: 54995; quals length: 3
at net.sf.samtools.BAMRecordCodec.encode(BAMRecordCodec.java:131)
at net.sf.samtools.BAMRecordCodec.encode(BAMRecordCodec.java:37)
at net.sf.samtools.util.SortingCollection.spillToDisk(SortingCollection.java:210)
at net.sf.samtools.util.SortingCollection.add(SortingCollection.java:150)
at net.sf.samtools.SAMFileWriterImpl.addAlignment(SAMFileWriterImpl.java:157)
at net.sf.picard.sam.MergeSamFiles.doWork(MergeSamFiles.java:207)
at net.sf.picard.cmdline.CommandLineProgram.instanceMain(CommandLineProgram.java:177)
at net.sf.picard.sam.MergeSamFiles.main(MergeSamFiles.java:84)


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