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-   -   HELP,why consider reads with 5' adaptor as contaminants? (http://seqanswers.com/forums/showthread.php?t=3647)

Euler 01-14-2010 11:50 PM

HELP,why consider reads with 5' adaptor as contaminants?
 
Hi,everyone.
I got a report about small RNA analysis.It is a Solexa sequencing results.Every read is 35 nt.
It first filter reads without 3' adaptor and then filter reads with 5' adaptor.
Can anybody explain it?Why keep 3' but leave 5'?


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