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-   -   Agilent Bioanalyser X axis doesn't size bands (http://seqanswers.com/forums/showthread.php?t=40219)

Sergioo 01-24-2014 01:50 AM

Agilent Bioanalyser X axis doesn't size bands
 
I raise this thread because I got difficult getting a way to change the X axis of Agilent Bionalyzer.
In fact, this axis is showing up in seconds and it won't change to size bands.
I saw a related post by pmiguel here http://seqanswers.com/forums/archive...hp/t-3338.html, but there is no answer there on how solve this.
Please note that both marker were well recognized and samples as well (samples has bigger than expect bands though).
I would like to hear from you a way out this.
Thanks:)

microgirl123 01-24-2014 08:06 AM

Just to check, you are using the toggle button to change the seconds to size? And it is not working? Is this all the time or just for one run? I have done one run that will not change - everything looks perfect, it just won't switch from seconds to size. If you use the region table, and put regions around the peaks you are interested in, you can tell the size from the table.

Sergioo 01-25-2014 03:33 AM

Quote:

Originally Posted by microgirl123 (Post 130608)
Just to check, you are using the toggle button to change the seconds to size? And it is not working? Is this all the time or just for one run? I have done one run that will not change - everything looks perfect, it just won't switch from seconds to size. If you use the region table, and put regions around the peaks you are interested in, you can tell the size from the table.

I did resolved it using the region table.
Thanks

kwaraska 02-03-2014 10:27 AM

Usually if it won;t toggle it is because of an error in one lane.

If you can remove that error, even if it means losing that sample, you can recovere the rest of the run.


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