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tickteng 04-23-2019 06:26 PM

Newbie: Primer with index
Hi everyone, kinda new to the whole sequencing. So I want to run multiple sample in minion. There is barcode provided by oxford nanopore, but i also would like to attach index to my pcr.

I am planning to make 18 or 22 bp of index.
The question is, is there any platform that you use to design index? or is it just randomly decided by you?

Marc_Jones 04-24-2019 12:53 AM

You could just add the index to the primers when you order them.


SQK-RPB004 or SQK-PBK004 depending on your experiment.

tickteng 04-24-2019 01:18 AM

hmm, not really like that. So beside the one provided by nanopore, I plan to attach some kind of index to the primer itself, so the result can be divided further in one barcode.

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