Hi,
We accidentally mixed a rapid library prep library (key pass GACT) with a general amplicon library (key pass TCAG) on the same run on a GS-Junior using LibL chemistry.
The machine will only detect the rapid library prep sequences (key pass GACT) and as such discards all the general amplicon library sequences (key pass TCAG) as no key pass.
I'm aware that in the system software manual is states: DO NOT mix samples prepared with the Rapid Library protocol with ones prepared with the FLX Standard protocol in the SAME region of the PTP device.
I was just wondering whether or not it would be possible to rescue these other sequences in some way bioinformatically, perhaps to override the use of the GACT keypass in favour of the TCAG keypass.
We accidentally mixed a rapid library prep library (key pass GACT) with a general amplicon library (key pass TCAG) on the same run on a GS-Junior using LibL chemistry.
The machine will only detect the rapid library prep sequences (key pass GACT) and as such discards all the general amplicon library sequences (key pass TCAG) as no key pass.
I'm aware that in the system software manual is states: DO NOT mix samples prepared with the Rapid Library protocol with ones prepared with the FLX Standard protocol in the SAME region of the PTP device.
I was just wondering whether or not it would be possible to rescue these other sequences in some way bioinformatically, perhaps to override the use of the GACT keypass in favour of the TCAG keypass.
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