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HTS 12-19-2009 08:18 AM

Sequencing bias on the Illumina platform
 
Hi,

I am trying to take a close look at the sequencing bias present in the Illumina platform and to think about ways to correct for it algorithmically, especially in the context of RNA-Seq. My main reference so far is the transcript length bias study by Oshlack and Wakefield. Does anyone know more recent/comprehensive studies and/or up-to-date information? Any helpful suggestions will be greatly appreciated. Thanks a lot!

-- Leo


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