SEQanswers (
-   Illumina/Solexa (
-   -   Prevention of index hopping in Illumina tech (

GenoMax 04-05-2018 07:51 AM

Prevention of index hopping in Illumina tech
Illumina has announced free adapter blocking agents which are supposed to help minimize index hopping.

pmiguel 04-05-2018 10:43 AM

Not clear what this is. Or what "minimizing index hopping" means. What fold reduction are they seeing with use of this reagent?

More info, including the manual here:

As always, I'm less worried about "free adapter" per se. It's the adapter strands annealed to full length library molecules that tend to escape clean-up.


JBKri 08-24-2020 03:20 AM

Anyone know if the Free Adapter Blocking Reagent is compatible with two-step PCR amplicon libraries? Anyone know how the reagent works?

All times are GMT -8. The time now is 01:26 PM.

Powered by vBulletin® Version 3.8.9
Copyright ©2000 - 2020, vBulletin Solutions, Inc.