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sebbol 01-12-2012 06:05 PM

HiC - crosslinking - glycine
hello. i have 2 questions.

1. what would be the best way to evaluate if the cross-linking went okay for Hi-C (chromosome interactions)?

2. i read different things about the temperature of quenching the formaldehyde with glycine. some do it at RT, some do it on ice or with ice-cold glycine. i am not a chemist. why would the temperature be of importance?

thanks for reading the post and your kind help!


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