SEQanswers

SEQanswers (http://seqanswers.com/forums/index.php)
-   Sample Prep / Library Generation (http://seqanswers.com/forums/forumdisplay.php?f=25)
-   -   HiC - crosslinking - glycine (http://seqanswers.com/forums/showthread.php?t=16823)

sebbol 01-12-2012 06:05 PM

HiC - crosslinking - glycine
 
hello. i have 2 questions.

1. what would be the best way to evaluate if the cross-linking went okay for Hi-C (chromosome interactions)?

2. i read different things about the temperature of quenching the formaldehyde with glycine. some do it at RT, some do it on ice or with ice-cold glycine. i am not a chemist. why would the temperature be of importance?

thanks for reading the post and your kind help!

Seb


All times are GMT -8. The time now is 09:32 AM.

Powered by vBulletin® Version 3.8.9
Copyright ©2000 - 2020, vBulletin Solutions, Inc.