hello to all
I want to sequence the Exome of a certain vertebrate with unknown genome (using SOLID).
I am going to extract RNA form tissue.
Does anyone have recommendation about which tissue (or tissues) to extract the RNA from, in order to get sequences that represent the Exome of the animal in the best way possible (in contrast to a tissue specific Exome - that might cause me to loose transcripts)
Thanks so much
Dan
I want to sequence the Exome of a certain vertebrate with unknown genome (using SOLID).
I am going to extract RNA form tissue.
Does anyone have recommendation about which tissue (or tissues) to extract the RNA from, in order to get sequences that represent the Exome of the animal in the best way possible (in contrast to a tissue specific Exome - that might cause me to loose transcripts)
Thanks so much
Dan
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