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-   -   Short read 1 on a Nova (http://seqanswers.com/forums/showthread.php?t=90597)

GW_OK 08-22-2019 12:38 PM

Short read 1 on a Nova
 
Anyone tried a read 1 less than 25 cycles on a Nova? We've done it before on Miseq and Nextseq but not on Nova. I'm wondering if there's anything weird about the patterned flowcell that would make it not work....

GenoMax 08-22-2019 12:59 PM

I don't immediately recall if we have done one on NovaSeq but have done so on patterned FC on HiSeq 4000. As long as you keep in mind that the quality scores are not properly calibrated until after cycle 25 it should work.


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