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-   -   MiSeqDx specifications (http://seqanswers.com/forums/showthread.php?t=40754)

alunem 02-10-2014 06:22 AM

MiSeqDx specifications
 
What are the specifications of the FDA-cleared version of the MiSeq. As I understood, it's a downgrade version of it (as it had to be locked since the beginning of FDA application processing), but what exactly?
- what is the expected ouput (number of reads, lenght...)
- what files are going to be available for the user? (are we going to have access to fastq files? bcl?)

Well, it's not easy to find these information on illumina's website.

Thanks!

GenoMax 02-10-2014 06:30 AM

http://res.illumina.com/documents/pr...instrument.pdf

alunem 02-10-2014 06:35 AM

Thanks, this answers my first question. Any idea about files available after sequencing processed?

GenoMax 02-10-2014 06:40 AM

You may want to check with your local FAS (who can in turn check with someone here in the US, if the instrument is not available in France).

My feeling (speculation) is the output may be some kind of a summary report saying such and such mutation is present/absent. No data may be directly accessible. Someone who has a MiSeqDX perhaps can provide additional information.

NextGenSeq 02-10-2014 09:13 AM

Wonder how they dealt with the well known carryover contamination issue. This would be a major problem for viral or tumor sequencing.


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