SEQanswers

SEQanswers (http://seqanswers.com/forums/index.php)
-   Ion Torrent (http://seqanswers.com/forums/forumdisplay.php?f=40)
-   -   barcode library for ion torrent (http://seqanswers.com/forums/showthread.php?t=58743)

linda2015 05-20-2015 11:28 AM

barcode library for ion torrent
 
Hi everybody, I am new in the NGS field. I want to ask a question. If I want to barcode my samples, should I do paired barcode or one side barcode, considering the reading length of ion torrent, If the fragment is more than 300bp, maybe there will be an issue to do paired barcod?

I want to do a pilot experiment to test my propose. but I hardly find literature regarding to my question.

I wonder if any of you have experience on this, can you share your thoughts?

Greatly appreciated!

maven 05-20-2015 09:52 PM

One barcode because Ion Torrent software doesn't look for barcodes on the other ends.

linda2015 05-21-2015 08:43 AM

Quote:

Originally Posted by maven (Post 172954)
One barcode because Ion Torrent software doesn't look for barcodes on the other ends.

thanks for your reply,
Another question is, I am not sure in which end to put barcode, in the beads end, is it P1 adapter and the other is A adpater? If I want to add barcode for sucessful read through, which end is better.

Thanks a lot


All times are GMT -8. The time now is 10:29 PM.

Powered by vBulletin® Version 3.8.9
Copyright ©2000 - 2020, vBulletin Solutions, Inc.