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Jieshu 02-06-2020 09:49 AM

Urgent: ATAC-seq barcoded primers
Hi guys,

I am new to ATAC-seq, I plan to try this method with Arabidopsis. I am going to order those primers from Supplementary Table 1 (Buenrostro et al., Nat Methods, 2013). For Ad1_noMX and bar-coded primers Ad2.1/2/3..., just order them as what they are? is there any modification for these bar-coded primers? I plan to order them with DESALT from Eurofins, could anyone give me some suggestions?



luc 02-06-2020 08:22 PM

I believe you can use standard barcoded Nextera primers instead. Preferentially UDI dual indexed versions of the Nextera primers.
Simple desalted oligos will be fine indeed.

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