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HTS 12-19-2009 09:18 AM

Sequencing bias on the Illumina platform

I am trying to take a close look at the sequencing bias present in the Illumina platform and to think about ways to correct for it algorithmically, especially in the context of RNA-Seq. My main reference so far is the transcript length bias study by Oshlack and Wakefield. Does anyone know more recent/comprehensive studies and/or up-to-date information? Any helpful suggestions will be greatly appreciated. Thanks a lot!

-- Leo

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