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-   -   3'UTR library or random primed cDNA library for quantification? (http://seqanswers.com/forums/showthread.php?t=2009)

Rosanne82 06-26-2009 06:27 AM

3'UTR library or random primed cDNA library for quantification?
 
I am quite new in the field of next generation sequencing and I hope to get some information and help here.
I want to do a RNA-Seq by using the Illumina platform to analyze the transcript profile of a specific tissue of a wildtype plant compared to a mutant. The aim is identifying a different transcription level of some genes as well as finding some rare genes. The organism I am working with is not sequenced yet, but it should be till the end of this year.
My question is which type of library is more suitable for quantification, a random primed cDNA library or a 3’UTR library, which proposed me a company. But what would be the advantage of a 3’UTR library?

Thank you very much!


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