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-   -   Fungal ITS amplicon sequencing (NexteraXT vs TruSeq Custom Amplicon) (http://seqanswers.com/forums/showthread.php?t=30932)

chapadosj 06-07-2013 08:59 AM

Fungal ITS amplicon sequencing (NexteraXT vs TruSeq Custom Amplicon)
 
We've just received our new MiSeq and we want to sequence fungal ITS amplicons from environmental samples. Previously, we used 454 fusion primers to tag samples before pooling. Should I design Illumina fusion primers and use the TSCA kit or would the NexteraXT kit be simpler? My boss is worried about shearing the amplicon.


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