Seqanswers Leaderboard Ad

Collapse

Announcement

Collapse
No announcement yet.
X
 
  • Filter
  • Time
  • Show
Clear All
new posts

  • FLASH : command not found

    Hi there,
    I'm a newbie in bioinformatics and I encounter a simple problem with FLASH (Fast Length Adjustment for SHort reads).
    I'm working on Mac OS X and followed the instructions to download, untar the tarball and compile using $make:
    $ make
    cc -O2 -Wall -std=c99 -D_GNU_SOURCE -D_FILE_OFFSET_BITS=64 -pthread -c -o flash.o flash.c
    cc -O2 -Wall -std=c99 -D_GNU_SOURCE -D_FILE_OFFSET_BITS=64 -pthread -c -o combine_reads.o combine_reads.c
    cc -O2 -Wall -std=c99 -D_GNU_SOURCE -D_FILE_OFFSET_BITS=64 -pthread -c -o fastq.o fastq.c
    cc -O2 -Wall -std=c99 -D_GNU_SOURCE -D_FILE_OFFSET_BITS=64 -pthread -c -o util.o util.c
    cc -pthread flash.o combine_reads.o fastq.o util.o -lz -o flash
    clang: warning: argument unused during compilation: '-pthread'

    But when I ran the command I got an error message:
    $ flash -o flashout_trimmed --interleaved-output trimmedR1.fastq trimmedR2.fastq
    -bash: flash: command not found

    Can anyone help with that?
    Thank you very much in advance!

  • #2
    you just need to add the directory flash was installed in to your .bash_profile.

    Alternatively you could copy it to something like /usr/local/bin/ or /usr/bin/

    What I do on my machine is to create local "bin" directory at just /home/username/bin which is added to my path in .bash_profile. I then make a symlink to the executable file, or just copy the executable over.

    Comment


    • #3
      Thank you!

      That worked, thank you very much!

      Comment

      Latest Articles

      Collapse

      • seqadmin
        Current Approaches to Protein Sequencing
        by seqadmin


        Proteins are often described as the workhorses of the cell, and identifying their sequences is key to understanding their role in biological processes and disease. Currently, the most common technique used to determine protein sequences is mass spectrometry. While still a valuable tool, mass spectrometry faces several limitations and requires a highly experienced scientist familiar with the equipment to operate it. Additionally, other proteomic methods, like affinity assays, are constrained...
        04-04-2024, 04:25 PM
      • seqadmin
        Strategies for Sequencing Challenging Samples
        by seqadmin


        Despite advancements in sequencing platforms and related sample preparation technologies, certain sample types continue to present significant challenges that can compromise sequencing results. Pedro Echave, Senior Manager of the Global Business Segment at Revvity, explained that the success of a sequencing experiment ultimately depends on the amount and integrity of the nucleic acid template (RNA or DNA) obtained from a sample. “The better the quality of the nucleic acid isolated...
        03-22-2024, 06:39 AM

      ad_right_rmr

      Collapse

      News

      Collapse

      Topics Statistics Last Post
      Started by seqadmin, 04-11-2024, 12:08 PM
      0 responses
      18 views
      0 likes
      Last Post seqadmin  
      Started by seqadmin, 04-10-2024, 10:19 PM
      0 responses
      22 views
      0 likes
      Last Post seqadmin  
      Started by seqadmin, 04-10-2024, 09:21 AM
      0 responses
      17 views
      0 likes
      Last Post seqadmin  
      Started by seqadmin, 04-04-2024, 09:00 AM
      0 responses
      49 views
      0 likes
      Last Post seqadmin  
      Working...
      X