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-   -   Library prep for 50ng input with TruSeq DNA- or RNAseq adapters? (http://seqanswers.com/forums/showthread.php?t=15372)

sehrrot 11-08-2011 04:38 PM

Library prep for 50ng input with TruSeq DNA- or RNAseq adapters?
 
Dear all,

I am going to process library preparation with 50 ng DNA. I am just concerning ratio of adapters:DNA input. Thus, I am considering to use adapters from TruSeq RNAseq kit, which has lower molarity adapters. Has anyone done library prep with lower DNA input?

pmiguel 11-09-2011 04:03 AM

Yes or you could just dilute your DNA TruSeq adapters 20x.

We have gone below 1 ug. Possibly to 100 ng, with no modifications to the protocol. But we generally size select prior to enrichment PCR. So adapter dimers are not that much of an issue.

--
Phillip


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