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seqgirl123 10-06-2009 06:30 PM

advantages and disadvantages of adapter barcoding?
 
Hello,
What are the advantages and disadvantages of barcoding adapters for multiplexing versus barcoding the PCR primers? Is adapter barcoding just another way to do multiplex?

BENM 10-07-2009 02:54 AM

"Although indexing has the obvious benefit of multiplexing samples within a run, DNA indexing offers two key additional advantages: direct measure of base-by-base error rate and reduction of arrayto-array or day-to-day variability."

--by David WCraig etc. al., Identification of genetic variants using bar-coded multiplexed sequencing, Nature Methods, Vol. 5, No. 10. (14 September 2008), pp. 887-893.


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