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-   -   alternatives to sera-mag bead purification (http://seqanswers.com/forums/showthread.php?t=4924)

peromhc 05-01-2010 02:20 AM

alternatives to sera-mag bead purification
 
Hi All,

Is anybody out there using something OTHER THAN the Illumina recommended sera-mag beads for mRNA purification?

The product works fine and all, but it takes so long, and there are many places to mess up.

I am interested in using a QIAGEN spin column or one of the Invitrogen products for direct mRNA isolation... Anybody using these (or another non-bead system) for Illumina mRNA sequencing?

peromhc 05-12-2010 09:03 AM

Well, I can now say at least that the QIAGEN Oligotrex mRNA purification kit does not work well- at least when following the protocol...

16% rRNA contamination... this is purifying from total RNA.


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