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NuGEN 06-17-2011 11:45 AM

New! Ovation RNA-Seq System V2 from NuGEN Technologies
We are pleased announce the release of our second generation kit for RNA-Seq applications. Along with the core features of our original system - low pg input of total RNA without the need for rRNA reduction or poly(A)+ selection, the Ovation RNA-Seq System V2 features:

Superior transcript coverage
Uniform read coverage across the transcriptome and exons
Improved detection of RefSeq genes

Streamlined workflow
cDNA product can be cloned directly by NGS library construction methods
Overall protocol shortened to 4.5 hours
Fewer kit components

Please see our product web page for more information about this product and examples of improved read coverage -


tk69 07-21-2011 09:38 PM

RNA seq Human CSF w/ Hiseq and pacbio, Nugen library
this site has been invaluable in my introduction to sequencing. It has allowed me to develop a lightly funded research plan with consensus evaluation of available technology including platforms and library prep. thanks to all, and as I begin this foray I will attempt to share my experiences with a metagenomic RNA seq of human CSF, using NUGEN and Hiseq followed by pac bio.

tk69 07-21-2011 09:46 PM

as far as I can tell, only Nugen can create an amplified library without poly A selection bias, with good coverage, and simultaneously exclude rRNA. miraculous actually. in searching for disease causing micro-organisms, entire classes of viruses (+ strand RNA viruses) and mycoplasma are not detected if one selects only polyA amplified transcripts for sequencing. I will be using this soon in coming weeks and post my experience.

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