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-   -   Effect of %AT content of genome on shearing (http://seqanswers.com/forums/showthread.php?t=15450)

Smriti 11-11-2011 01:12 PM

Effect of %AT content of genome on shearing
 
2 Attachment(s)
Hi There,

Can anyone help me finding an article/study where effect of %AT content has been related to the shearing efficiency. I sheared Lambda and Plasmodium DNA with same settings. Surprisingly, Plasmodium sheared much faster. Also, I got a wavy slope towards lower base pairs in Plasmodium as compared to Lambda DNA. I am attaching the bioanalyzer curves of both DNA here. Can anyone help me in explaining this behavior. I know that Plasmodium DNA has 80%AT content compared to Lambda (50%).


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