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-   -   qPCR to validate/quantify Nextera library (http://seqanswers.com/forums/showthread.php?t=16204)

mikmz 12-14-2011 01:26 AM

qPCR to validate/quantify Nextera library
 
Hi,

I wish to validate my Nextera DNA library by qPCR.

Is this done merely by comparing against a control DNA library, i.e. the one that comes with the kit?

Is it possible to quanitify in absolute terms by qPCR or is this done with the BioAnalyzer?

Thanks,

M


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