SEQanswers

SEQanswers (http://seqanswers.com/forums/index.php)
-   Illumina/Solexa (http://seqanswers.com/forums/forumdisplay.php?f=6)
-   -   What volume of adapters do you get in the nextera XT kits? (http://seqanswers.com/forums/showthread.php?t=85491)

NGSMicro 11-07-2018 06:01 AM

What volume of adapters do you get in the nextera XT kits?
 
Hello,

At our lab we have a custom protocol for adding indices to our amplicons, using only 5uL of each index when using the "Nextera XT Index Kit (24 indexes, 96 samples)".

I want to update this to use the "IDT® for Illumina® Nextera™ DNA UD Indexes Set A (96 Indexes, 96 Samples)" kit, but I can't find out how much (approximately) you get in each kit for each pair of indices.

Has anyone bought this kit?

nucacidhunter 11-08-2018 02:47 AM

10 ul with some overage in each well which contains pool of two primers.

NGSMicro 11-08-2018 03:04 AM

Thank you, most appreciated.


All times are GMT -8. The time now is 06:37 AM.

Powered by vBulletin® Version 3.8.9
Copyright ©2000 - 2020, vBulletin Solutions, Inc.