Tweet
Hey, I'd like to compare the upregulation / downregulation of multiple transcriptomes (~ 5) when exposed to the same environmental stimulus. What I'm wondering is whether there are existing perl scripts / software programs to do this. Does anyone know? I'm willing to cook 'em up but don't want to re-make the wheel if I don't have to. Thanks.
-
-
In order to stimulate discussion related to your question it would be helpful to know more details.
Describe your data: sequencing platform? Number of reads per library? Paired or single end? Read length?
Experimental design: You say five conditions, but are paired comparisons available (treated vs untreated)? Do you have biological replicates?
Analysis goals? Expression analysis only? Gene level only or alternative expression as well?
-
The field of epigenetics has traditionally concentrated more on DNA and how changes like methylation and phosphorylation of histones impact gene expression and regulation. However, our increased understanding of RNA modifications and their importance in cellular processes has led to a rise in epitranscriptomics research. “Epitranscriptomics brings together the concepts of epigenetics and gene expression,” explained Adrien Leger, PhD, Principal Research Scientist...04-22-2024, 07:01 AM -
Proteins are often described as the workhorses of the cell, and identifying their sequences is key to understanding their role in biological processes and disease. Currently, the most common technique used to determine protein sequences is mass spectrometry. While still a valuable tool, mass spectrometry faces several limitations and requires a highly experienced scientist familiar with the equipment to operate it. Additionally, other proteomic methods, like affinity assays, are constrained...04-04-2024, 04:25 PM
Comment