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STAR alignment with multiple fq files
I am trying to do RNAseq PE alignment using STAR. It works for one pair of fq files:
$ STAR --runThreadN 30 --genomeDir /srv/STAR_genome/human/ --readFilesIn /../RNA4_1_R1.fastq.gz /../RNA4_1_R2.fastq.gz --readFilesCommand zcat However, I have multiple fq files for one sample and I need to align them into one sam file. When I tried to align all together, it returns 'Segmentation fault (core dumped)': $ STAR --runThreadN 30 --genomeDir /../STAR_genome/human/ --readFilesIn /../RNA4_1_R1.fastq.gz /../RNA4_1_R2.fastq.gz /srv/RNA4_2_R1.fastq.gz /../RNA4_2_R2.fastq.gz --readFilesCommand zcat Segmentation fault (core dumped) Could STAR deal with multiple pairs? What am I missing, pls? |
Sorry I figured it out. But I don't know how to delete the post.
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Could you please share? Thank you
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