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DNADEB 01-22-2013 07:08 AM

Single Cell Ampliseq?
We are developing a workflow for using the Ampliseq Cancer Panel starting with DNA from single cells. We plan to use WGA but wonder if someone could recommend a kit. Genomiphi for example requires 10 ngs. A single cell will have about 6 pgs. Any advice?

cliffbeall 01-22-2013 01:26 PM

The following paper has a method to purify the Phi29 polymerase away from DNA contamination, which can be a problem when amplifying low concentrations of DNA. I am not sure if there is a commercial product yet.

Blainey, P. C. and S. R. Quake (2011). "Digital MDA for enumeration of total nucleic acid contamination." Nucleic Acids Research 39(4) e19.

kwaraska 01-23-2013 06:36 AM

Qiagen has just introduced a product that is a single cell WGA.

Don't have any experience with it but might be a good starting point.

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