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-   -   RNA Sequencing at doctor's office (http://seqanswers.com/forums/showthread.php?t=80433)

fuellen 02-01-2018 10:23 PM

RNA Sequencing at doctor's office
 
Hello!
I'm looking for feasible scenarios that would enable gene expression data analyses at a doctor's office, probably based on a blood sample. It seems that sample processing is too sophisticated to be done there, so samples would have to be frozen and shipped. Question: How long can blood samples, after minimal processing, be stored at -18 degrees Celsius (or at +4 degrees, possibly) so that they may then be sent in bulk (eg on dry ice) to a sequencing facility?
Thanks for any hints!

HESmith 02-02-2018 05:31 AM

Expression profiles can change in minutes, so I'd recommend adding a stabilization component (e.g., Qiagen's RNAprotect) immediately after collection. At that point, the samples would be stable for weeks at 4˚ and indefinitely if frozen.

GW_OK 02-09-2018 07:43 AM

Is there a reason you can't draw into a PAXgene tube?


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