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  • How to clip multiple adapters in Penn State Version Galaxy? Thanks.

    Hi All,

    In " Clip adapter sequences" option of Penn State Version Galaxy, I choose "Enter custom sequence" under "Source", and I can only enter One custom clipping sequence. So how can I enter another 3 adapter sequences which I want to clip.

    In addition, In "What it does" it shows:
    This tool clips adapters from the 3'-end of the sequences in a FASTA/FASTQ file.

    So how could i do if I have the adapter sequences like this:

    5' TACACTCTTTCCCTACACGACGCTCTTCCGATCT
    5 'AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTA
    5' GACGGCATACGAGCTCTTCCGATCT
    5' AGATCGGAAGAGCTCGTATGCCGTC

    Thanks very much for any response!

  • #2
    Run the tool 4 times if you have 4 adapters

    Or install your own Galaxy instance and a more flexible tool, there are some on the Galaxy Tool Shed - http://toolshed.g2.bx.psu.edu/

    Comment


    • #3
      Thanks a lot maubp, Running 4 times is really time-consuming

      Originally posted by maubp View Post
      Run the tool 4 times if you have 4 adapters

      Or install your own Galaxy instance and a more flexible tool, there are some on the Galaxy Tool Shed - http://toolshed.g2.bx.psu.edu/

      Comment

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