SEQanswers

SEQanswers (http://seqanswers.com/forums/index.php)
-   General (http://seqanswers.com/forums/forumdisplay.php?f=16)
-   -   SNP array analysis (http://seqanswers.com/forums/showthread.php?t=92448)

chobeich 01-09-2020 10:25 PM

SNP array analysis
 
I there!!

A friend has passed me the data of an Infinium Omni5-4 array. In short, they have applied two different mutagens to a cell line. I have passed a data set that contains:
3 mother samples (MS)
3 samples mutagen 1 (M1)
3 samples mutagen 2 (M2)
Its objective is to identify the mutagenic potential of each mutagen.
After googeling, I think what I need to do is a Manhattan plot to identify the chromosomal regions that have mutated the most for each mutagen.
What I have done at the moment is:
1 Genotyping callin, using the Genotyping Module of Genome Studio
2 Establish the reference set: SNP's 100% call and with the same genotype in all MS.
3 Assign each SNP of samples M1 and M2 a 1 if the genotype has varied from the reference or a 0 if it has not. The SNPs of the M1 and M2 samples that are not in the reference set or that are not 100% called have been discarded.
I have done all this with perl. What I now have is a text file with the following structure:
SNP_1 SNP_2 ...
M1.1 1 0
M1.2 1 1
.
.
M2.3 1 0

And that's all folks!!, I have no idea what I should do now.
Any help will be welcome because, as you may deduced, I have no idea of working with arrays or statistics.
Thank you

chobeich 01-29-2020 01:23 AM

Hi Vasudev,
I'm afraid this is not an array analysis, at least not complete.
The part of the analysis of results, that I hope someday someone completes, is missing.
In addition, until someone with experience in the analysis of arrays is pronounced, this should be interpreted as a noob's attempt to interpret data with which he had never worked before.
Anyway, thanks for your comment.


All times are GMT -8. The time now is 12:56 AM.

Powered by vBulletin® Version 3.8.9
Copyright ©2000 - 2020, vBulletin Solutions, Inc.