hello everyone , i'am sample prepation of mRNA, and i have a question about adapter ligation to the dsCDNA:
the protocol of illuminate write: incubate the sample at room temperature for 15 minutes.
as we know we usually make a ligation action overnight, i wonder if 15 minutes is enough for ligation reaction?
thanks! hopes your join!
the protocol of illuminate write: incubate the sample at room temperature for 15 minutes.
as we know we usually make a ligation action overnight, i wonder if 15 minutes is enough for ligation reaction?
thanks! hopes your join!
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