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torbean 11-12-2015 03:08 AM

Buy sheared DNA
 
Hi all,

I don't have Covaris, and need some sheared control DNA. Ideally, broad range, so I can size select. Anybody knows commercially available sheared DNA (available in EU)?

Thank you.

nucacidhunter 11-12-2015 05:28 PM

You cam make your own fragmented DNA by using a cheap source of DNA and 6 or 4 cutter or combination of restriction enzymes. Other option is using NEB Fragmentase product: https://www.neb.com/products/m0348-n...na-fragmentase

I do not think there is a high demand for fragmented DNA, so I never have heard of it. A commercial option is buying SageScience control DNA for Pippin systems. They are restriction fragments and you would need a clean up to remove their loading buffer before using for size selection experiment. http://www.sagescience.com/product-s...-prep-support/

SNPsaurus 11-12-2015 08:51 PM

Many places sell sheared salmon sperm DNA as a blocking reagent. I'm not totally sure the size range is in the range you desire, but it might be!

nucacidhunter 11-12-2015 10:12 PM

Thanks SNPsaurus for reminding Salmon DNA. The product size varies from vendor to vendor. ThermoFisher product size is ≤2,000 bp, Trevigen is 80-500 bp and Agilent one is 300-2000 bp. Other suppliers might have different size range.

torbean 11-12-2015 11:25 PM

oh that' a good idea really! Thanks. I was thinking about fragmentase or some ZnCl2 digestion or so, but wanted to keep it simple.

davidluthian 03-11-2019 10:30 PM

Quote:

Originally Posted by SNPsaurus (Post 184619)
Many places sell sheared salmon sperm DNA as a blocking reagent. I'm not totally sure the size range is in the range you desire, but it might be!

Yes this is true


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