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niceday 10-12-2011 06:00 AM

truseq exome enrichment
 
Sorry if this question has already been posted.

Has anyone tried to get around truseq's gel size selection when preparing libraries for exome enrichment?

We would normally use a 6 minute fragmentation as quoted by agilent but this gives post exome enrichment libraries of around 300bp or slightly over. Truseq want libraries to be 100bp larger.
We are trying to automate and remove gel size selection if possible.

Paolo oxford 11-04-2011 03:52 AM

Hi, have you checked the new version of the truseq kit, they removed gel size selection. the fragmentation they recommend goes with the capture probe design which is substantially different from agilent


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