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-   -   Sequencing Library Construction of Solexa (http://seqanswers.com/forums/showthread.php?t=16148)

chloe_giselle 12-10-2011 09:53 PM

Sequencing Library Construction of Solexa
 
Hi All,
1) What is Solexa?

ECO 12-10-2011 10:07 PM

Please search before posting. This is the most basic of questions.

pmiguel 12-11-2011 12:56 PM

Quote:

Originally Posted by chloe_giselle (Post 59274)
Hi All,

I am new to NGS and I am still trying hard to understand Solid and Solexa.

I defined "sequencing library" as the library prepared in the wetlab for being sequenced by a sequencer.

1) What are the methods used for constructing sequencing library in Solexa?

Hi Chloe,
Your very first post and already being chastised by the forum's owner. Not the best beginning for you.

There isn't really a "Solexa" any more. The company was purchased by Illumina some years ago and use of the old name "Solexa" has fallen by the wayside.
This question is too vague. There are numerous methods for constructing libraries for use on an Illumina sequencer.
Quote:

Originally Posted by chloe_giselle (Post 59274)
2) How about for Solid? What are the differences in the construction between these two?

This question is of some interest to me. My answer probably won't help you get a good grade on your home work, if that is where this question comes from. Because I doubt whomever wrote the question knows the actual answer.

They differ from library construction for the SOLiD mainly for historical reasons. With a little effort you could use Illumina library construction kits for constructing SOLiD libraries and vice-versa.

The only real distinction between a library made for a SOLiD and one made for an Illumina sequencer are that they use difference template PCR (ePCR for SOLiD, cluster/bridge PCR for Illumina) adapter sequences. Especially the sequence of the primer actually attached to the substrate (bead or flowcell) is instrument specific.

In addition, Illumina typically employes a single, partially double-stranded Y-adapter for library construction, whereas the older, two adapter approach is still used by SOLiD.
Quote:

Originally Posted by chloe_giselle (Post 59274)
Please advise me. I need to know this urgently so that I can quickly work on my experiment. Many thanks in advance :)

There you go: enough rope to hang yourself.

--
Phillip


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