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Sliding window/genome coverage from pileup files?
I have SOLiD whole genome sequence data from different yeast strains (S. cerevisiae) that have been processed using samtools.
I have used samtools pileup and have .pileup file for all my yeast strains (10 column pileup format). I would like to visualise/work out (say, maybe with a sliding window approach) not only the % genome coverage (compared to the standard S288C S. cerevisiae reference sequence) but also where the coverage is and what read depth. Does anyone have any suggestions? Thanks in advance |
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