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floraliu 06-21-2011 02:03 PM

Hi, one question about RNA-Seq library prep
 
Hi all,

I'm new in this field and want to learn something here.:)

I'm doing RNA-Seq library prep, we need to get good libraries from small starting total RNA material. When i tried to use 500ng total RNA to start, i usually couldn't get more than 1ng of purified cDNA. Does anyone have some suggestions on how to get more cDNA?


Thanks
-Flora


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