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stasi 02-17-2013 04:41 AM

elution buffer Question
I work with illumina (TruSeq) kits for RNA and DNA sample preparation.
Unfortunately, I spilled the elution buffer (for RNA prep). I try to avoid ordering a complete kit. Maybe someone knows what buffer can replace it or maybe know another kit (cheaper) containing the elution buffer.

biznatch 02-17-2013 05:34 AM

You might be able to order just that elution buffer from illumina. We use Qiagen kits for lots of things and have ordered individual buffers on several occasions.

pmiguel 02-18-2013 05:13 AM

In the US Illumina will generally replace a component if you accidentally spill it. Call Tech support and/or your sales rep for help.


kcchan 02-22-2013 03:38 PM

As pmiguel said, Illumina is generally very good about replacing reagents. If you're in a bind you can also just use Tris-HCl or even nuclease free water for the bead elution.

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