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  • Single-cell analysis: from DNA to RNA world

    Hi there,

    I'm new to this community, so this is to say hello and - at the same time - thanks to those providing helpful hints and information...

    I was doing single/rare-cell DNA analysis and joined a team working on single-cell RNA issues...
    ... now that I got introduced to the RNA world (which caused some extra adaptation in the lab as my DNA-lab-reference-system is kind of resisting to hand over to RNA) I more or less feel comfortable and can dig into things.

    The reason for being here is that I'm working on single-cell RNA sequencing which is why I started to follow the Smart-Seq2 protocol published earlier this year. Happy about the info I already got by surfing through the posts I might come up with one or two myself...

    Looking forward to interact ...

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  • seqadmin
    Current Approaches to Protein Sequencing
    by seqadmin


    Proteins are often described as the workhorses of the cell, and identifying their sequences is key to understanding their role in biological processes and disease. Currently, the most common technique used to determine protein sequences is mass spectrometry. While still a valuable tool, mass spectrometry faces several limitations and requires a highly experienced scientist familiar with the equipment to operate it. Additionally, other proteomic methods, like affinity assays, are constrained...
    04-04-2024, 04:25 PM
  • seqadmin
    Strategies for Sequencing Challenging Samples
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    Despite advancements in sequencing platforms and related sample preparation technologies, certain sample types continue to present significant challenges that can compromise sequencing results. Pedro Echave, Senior Manager of the Global Business Segment at Revvity, explained that the success of a sequencing experiment ultimately depends on the amount and integrity of the nucleic acid template (RNA or DNA) obtained from a sample. “The better the quality of the nucleic acid isolated...
    03-22-2024, 06:39 AM

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