SEQanswers

SEQanswers (http://seqanswers.com/forums/index.php)
-   Sample Prep / Library Generation (http://seqanswers.com/forums/forumdisplay.php?f=25)
-   -   Majority of RNA-Seq library is mitochondrial RNA (http://seqanswers.com/forums/showthread.php?t=10824)

brain_food 04-18-2011 11:35 AM

Majority of RNA-Seq library is mitochondrial RNA
 
Hi everyone,

We're currently attempting to make Illumina sequencing libraries with Epicentre's Ribo-Zero. Before sequencing, we TOPO-cloned our libraries and sequenced to clones (~20 per library) to get an idea of our libraries' diversity. Much to our dismay, we have found that a large portion of our clones (60-70%) map back to mitochondrial DNA. The predominant sequence bears a great deal of homology to the human gene Mt-rnr2-l (our libraries are from mouse). Has anyone else had similar difficulties with mitochondrial RNA? I've seen a few posts on this site where a higher mitochondrial RNA quantity has been referenced, but none addressing this problem specifically. I appreciate any help you can offer. Thanks in advance.


All times are GMT -8. The time now is 06:05 AM.

Powered by vBulletin® Version 3.8.9
Copyright ©2000 - 2020, vBulletin Solutions, Inc.