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salkh 12-14-2011 11:59 PM

RiboZero for non-human samples
 
Does anyone use the RiboZero kits for rRNA depletion of non-human/mouse/rat samples? I mean vero, BHK, or CHO etc. cells.

/Serge

epibio 12-15-2011 07:43 AM

Mammalian cultured cell lines should work well with the Ribo-Zero H/M/R kit. For other types of samples, please see our species compatibility list

RayLangley 01-05-2012 09:21 AM

I recently used on lung RNA from cyno maqaques samples and it worked great.

Emma71 10-12-2016 12:06 PM

I have problem with mouse.

dwong 11-04-2016 09:28 AM

For specific organism depletion, have you considered using NuGEN’s model organism kits utilizing our InDA-C technology? For non-model organisms, we offer customization. The way this works is that you provide us with information on the organism of choice, and sequences which you want depleted (and this is not limited to ribosomal RNA, but other highly abundant transcripts) and we design specific probes to target these transcripts. For the more standard organism such as mouse and rat, we have off the shelf kits available:

http://www.nugen.com/products/ngs/ov...odel-organisms

Darren


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