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-   -   Sequencing through high GC content repeat region (http://seqanswers.com/forums/showthread.php?t=20982)

anna6832 06-19-2012 11:41 AM

Sequencing through high GC content repeat region
 
I've sequenced a viral genome with the Illumina HiSeq but have some holes where there are repeat regions. The whole genome is 73% GC-rich so the repeats make it even harder to get through. I want to re-sequence these areas, does anyone have any suggestions or methods that helps get accurate sequence data from repeats with high GC content?


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