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-   -   How to work with biological replicates (http://seqanswers.com/forums/showthread.php?t=28919)

babi2305 04-02-2013 02:17 PM

How to work with biological replicates
 
Hello everyone,

I am working with small-RNA(~50ntds) cancer samples, RNA-seq data. I have tumors and normal cell samples with biological replicates of each sample. Using bed-tools coverageBed I counted the read distribution in genomic regions as well as in 10kb interval chr regions and identified the correlation. My problems are:

-All my biological replicates are showing good correlation except one (good ones=0.98,0.96 etc bad one =~0.6) so now I want to know the methods to study for correlation of biological replicates.

-How can I compare and extract meaningful regions from the above badly correlated replicate.

-Can anybody tell the correct method for identifying correlation and coverage depths of biological replicates?

Please help & tell me how should I proceed. :rolleyes:

Thanks.


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